Apoptosis repressor with CARD Antibody
Size
0,1 mgCatalog no#
PA1793Price
320 EURAnalyses
WB
Gene Name
NOL3
Raised in
rabbit
Uniprot ID
O60936
French translation
anticorps
Product form
freeze-dried
Clone
Polyclonal antibody
Protein Name
Nucleolar protein 3
Clonality
Polyclonal antibody
Type of the antibody
IgG polyclonal antibody
Purification
Immunogen affinity purified.
Target antigen
Apoptosis repressor with CARD
Reacts with species:
human, rat Predicted to work with: mouse
Description
This 1 is suited for programmed cell-death studies.
Gene Full Name
nucleolar protein 3(apoptosis repressor with CARD doma)
Product configuration
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Solubilization
The powdered antibody should be dissolved in 0.2 ml of distilled water to achieve final concentration of 500ug/ml
Properties
If you buy Antibodies supplied by boster they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Tips
The Apoptosis repressor with CARD Antibody did not cross-reacted with other proteins during the test procedures. This antobdy is intended to be used for research analyses and it is not applicale for in vitro diagnostics.
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human Apoptosis repressor with CARD(91-106aa WDWQHVGPGYRDRSYD), identical to the related rat sequence, and different from the related mouse sequence by one amino acid.
Synonyms
Apoptosis repressor with CARD antibody|ARC antibody|Muscle enriched cytoplasmic protein antibody|Muscle-enriched cytoplasmic protein antibody|MYC antibody|MYP antibody|Nol3 antibody|NOL3_HUMAN antibody|NOP antibody|Nop30 antibody|Nucleolar protein 3(apoptosis repressor with CARD domain) antibody|Nucleolar protein 3 antibody|Nucleolar protein of 30 kDa antibody
Storage condtions
Keep the Apoptosis repressor with CARD Antibodyat minus twenty degrees Celsius for 1 year. The ready-to-use solutions can be stored at four degrees Celsius for a month. Our specialsits recommend to freeze the aliquotes at minus twenty degrees Celsius for long-term application. Multiple procedures of freezing and thawing influence the specifity and reactivity of the antibody in a negative way.
Related articles
1. Abmayr, S., Crawford, R. W., Chamberlain, J. S.Characterization of ARC, apoptosis repressor interacting with CARD, in normal and dystrophin-deficient skeletal muscle.Hum. Molec. Genet. 13: 213-221, 2004. 2. Koseki, T., Inohara, N., Chen, S., Nunez, G.ARC, an inhibitor of apoptosis expressed in skeletal muscle and heart that interacts selectively with caspases.Proc. Nat. Acad. Sci. 95: 5156-5160, 1998. 3. Stoss, O., Schwaiger, F.-W., Cooper, T. A., Stamm, S.Alternative splicing determines the intracellular localization of the novel nuclear protein Nop30 and its interaction with the splicing factor SRp30c.J. Biol. Chem. 274: 10951-10962, 1999.
Background
NOL3(Nucleolar protein 3), also known as ARC, NOP30, CARD2 and MYP, is a protein that in humans is encoded by the NOL3 gene. NOL3 has been shown to interact with SFRS9 and Caspase 8. By genomic sequence analysis, Stoss et al.(1999) determined that the NOL3 gene, which encodes NOP30 and MYP and which they called NOP, is composed of 4 exons. The alternative 5-prime splice site that generates the 2 isoforms is located in exon 2. It is reported that expression of the ARC cDNA encoding the smaller transcript inhibited apoptosis in a dose-dependent manner when coexpressed with CASP8 but not when coexpressed with CASP9. ARC also inhibited apoptosis induced by stimulation of CD95/FAS, tumor necrosis factor receptor-1, and TRAMP/death receptor-3. Enzymatic analysis showed that ARC inhibits the enzymatic activity of CASP8. Immunoprecipitation and immunoblot analysis indicated that ARC interacts with CASP2 and CASP8 through its N-terminal death effector domain but does not interact with CASP1, CASP3, or CASP9.