Apoptosis-Inducing Factor Antibody (Mitochondrial marker)
Size
0.1mgCatalog no#
R31356Price
389 EURUniprot #
O95831
Category
Antibody
French translation
anticorps
Conjugation
Unconjugated
Tissue
mitochondrial
Purity
Antigen affinity
Clone
Polyclonal antibody
Tested applications
WB, IHC-P, IHC-F, ICC
Form
Antigen affinity purified
Clonality
Polyclonal (rabbit origin)
Host animal
Rabbit (Oryctolagus cuniculus)
Recognised antigen
Apoptosis-Inducing Factor (Mitochondrial marker)
Concentration
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Intented use
This Apoptosis-Inducing Factor antibodyis to be used only for research purposes and not for diagnostics..
Recommended dilutions
Western blot: 0.5-1ug/ml,IHC (Paraffin): 0.5-1ug/ml,IHC (Frozen): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml
Properties
If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Immunogen
An amino acid sequence from the C-terminus of human AIFM1 (EQHEDLNEVAKLFNIHED) was used as the immunogen for this Apoptosis-Inducing Factor antibody (100% homologous in human, mouse and rat).
Species reactivity
Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
Notes
The stated application concentrations are suggested starting amounts. Titration of the Apoptosis-Inducing Factor antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Storage
After reconstitution, the Apoptosis-Inducing Factor antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
Additional description
This 1 is suited for programmed cell-death studies.Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.
Description
Apoptosis-inducing factor 1, mitochondrial, also known as AIF or PDCD8 is a protein that in humans is encoded by the AIFM1 gene. This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Mutations in this gene cause combined oxidative phosphorylation deficiency 6, which results in a severe mitochondrial encephalomyopathy. A related pseudogene has been identified on chromosome 10.