Apoptosis inhibitor 5 Antibody

Size

0.1mg

Catalog no#

R30177

Price

389 EUR

Uniprot #

Q9BZZ5

Category

Antibody

French translation

anticorps

Conjugation

Unconjugated

Purity

Antigen affinity

Clone

Polyclonal antibody

Tested applications

WB, IHC-P, IHC-F, ICC

Recognised antigen

Apoptosis inhibitor 5

Form

Antigen affinity purified

Clonality

Polyclonal (rabbit origin)

Host animal

Rabbit (Oryctolagus cuniculus)

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Intented use

This Apoptosis inhibitor 5 antibodyis to be used only for research purposes and not for diagnostics..

Recommended dilutions

Western blot: 0.5-1ug/ml,IHC (Paraffin): 0.5-1ug/ml,IHC (Frozen): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml

Properties

If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Immunogen

An amino acid sequence from the C-terminus of human Apoptosis inhibitor 5 (amino acids 487-504 of isoform b, KYSSNLGNFNYERSLQGK) was used as the immunogen for this Apoptosis inhibitor 5 antibody.

Species reactivity

Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.

Notes

The stated application concentrations are suggested starting amounts. Titration of the Apoptosis inhibitor 5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Storage

After reconstitution, the Apoptosis inhibitor 5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.

Description

Many growth factors and cytokines act as cellular survival factors by preventing programmed cell death(apoptosis). Apoptosis inhibitor 5 (API5) is an antiapoptotic factor which may have a role in protein assembly. The API5 gene to chromosome 11p12 based on an alignment of the API5 sequence with the genomic sequence It is a critical determinant of E2F1-induced apoptosis, acting downstream of E2F to suppress E2F-dependent apoptosis without generally blocking E2F-dependent transcription.

Additional description

This 1 is suited for programmed cell-death studies.Tissue, pathway, proteinase, peptidase, protease ,acrosin, lipoprotein, activator, caspase, trypsin, papain, esterase inhibitors are proteins or receptor ligands or receptor antagonists that bind to an enzyme receptor and decreases its activity. Since blocking an enzyme's activity can kill a pathogen or correct a metabolic imbalance, many drugs are enzyme inhibitors. Not all receptor antagonist that bind to enzymes are inhibitors; enzyme activator ligands or agonists bind to enzymes and increase their enzymatic activity, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme.