Cytochrome c Releasing Apoptosis Assay Kit
Size
100 assaysCatalog no#
K257-100Price
611 EURKit's other name
none
Storage condition
-20°C
Shipping condition
gel pack
Maximum time can be stored
12 months
Species reactivity
see datasheet
Samples tested
Cells and tissues
Category
Apoptosis Assay Kit
Test
Biovision supplies other types of Assays as 1.
Description
This 1 is suited for programmed cell-death studies.
Contents
• Mitochondria Extraction Buffer • 5X Cytosol Extraction Buffer • DTT (1 M) • 500X Protease Inhibitor Cocktail • Anti-Cytochrome c mouse mAb (0.2 mg/ml)
Applications
Effective means for detecting cytochrome c translocation from mitochondria into cytosol during apoptosis. The kit provides an easy:to:use procedure and reagents for separating a mitochondria:enriched fraction from cytosol.
Kit's benefits
• Simple procedure; takes ~3 hours • Fast and convenient • Cytochrome c releasing from mitochondria into cytosol is then determined by Western blotting using the cytochrome c antibody provided in the kit. The procedure is simple and straightforward. No ultracentrifugation is required.
Highlights
• Detection method: Western blotting • Species reactivity: Mammalian • Applications: Effective means for detecting cytochrome c translocation from mitochondria into cytosol during apoptosis. The kit provides an easy:to:use procedure and reagents for separating a mitochondria:enriched fraction from cytosol.
Kit's description
Cytochrome c plays an important role in apoptosis. The protein is located in the space between the inner and outer mitochondrial membranes. An apoptotic stimulus triggers the release of cytochrome c from the mitochondria into cytosol where it binds to Apaf-1. The cytochrome c/Apaf-1 complex activates caspase-9, which then activates caspase-3 and other downstream caspases. BioVision’s Cytochrome c Releasing Apoptosis Assay Kit provides an effective means for detecting cytochrome c translocation from mitochondria into cytosol during apoptosis. The kit provides unique formulations of reagents to isolate a highly enriched mitochondria fraction from cytosol. The procedure is so simple and easy to perform, no ultracentrifugation is required and no toxic chemicals are involved. Cytochrome c releasing from mitochondria into cytosol is then determined by Western blotting using the cytochrome c antibody provided in the kit.