CaspSCREEN Flow Cytometric Apoptosis Detection Kit

Size

100 assays

Catalog no#

55R-1301

Price

688 EUR

Shipping conditions

Blue Ice

Category

Research kit

Storage

Store at -20 deg C

Tested for

FC; Functional Assay

Area of research

Cell Cycle & Cell Death

Additional Information

CaspSCREEN is a registered trademark

Form & Buffer

Assay Kit containing the folllowing: D₂R Reagent, DTT (1 M), D₂R Incubation Buffer

Description

This 1 is suited for programmed cell-death studies.The detections of the targets with this kit is a type of test that can be performed on any target containing biological samples after clean up of interfering agents. The assay must be performed following the protocol.

Test

Flow cytometry uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal.