Annexin V Apoptosis Detection Kit with PI-Biotin

Size

100 TEST

Catalog no#

ANXVKB-100T

Price

317 EUR

Conjugate

Biotin

Conjugation

Biotinylated

Expiration

After 1 year

Reactivity

All mammalian

Application

Flow Cytometry Method

Remark

For Recherche Use Only.

Other name

Annexin A5 Apoptosis Detection Kit

Storage

2-8°C, store in dark, do not freeze

Formulation

Aqueous buffered solution containing protein stabilizer, and ≤0.09% sodium azide (NaN3)

Properties

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.

Description

Annexin V Apoptosis Detection Kit with PI-Biotin is a kit used for the identifacation and detection of apoptotic and necrotic cells, this Kit was conjugated with Biotin format and reported for use in flow cytometric analysis, it reacts with Annexin V proteins, with Propidium Iodide Solution (PI) helps to make the difference between apoptic cells and necrotic, used also as a dead cell exclusion dye.

Additional description

This Annexin V calcium dependent phospholipid binding protein will be downstream of BCL2 pathway and at caspase apoptotic level. Immunostep has more cell death kits and antibodies.This 1 is suited for programmed cell-death studies.The detections of the targets with this kit is a type of test that can be performed on any target containing biological samples after clean up of interfering agents. The assay must be performed following the protocol.