FITC

After 1 year

All mammalian

Anti-FITC Antibody

Flow Cytometry Method

For Recherche Use Only.

Annexin A5 Apoptosis Detection Kit

2-8°C, store in dark, do not freeze

Aqueous buffered solution containing protein stabilizer, and ≤0.09% sodium azide (NaN3)

Annexin V Apoptosis Detection Kit with 7AAD-FITC is a kit used for the identifacation and detection of apoptotic and necrotic cells, this Kit was conjugated with FITC format and reported for use in flow cytometric analysis, it reacts with Annexin V proteins, with 7-amino-actinomycin D (7-AAD) helps to make the difference between apoptic cells and necrotic, used also as a dead cell exclusion dye.

This Annexin V calcium dependent phospholipid binding protein will be downstream of BCL2 pathway and at caspase apoptotic level. Immunostep has more cell death kits and antibodies.This 1 is suited for programmed cell-death studies.The detections of the targets with this kit is a type of test that can be performed on any target containing biological samples after clean up of interfering agents. The assay must be performed following the protocol.

This Immunostep Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Immunostep FITC is highly sensitive to pH extremes.