Annexin V-FITC Apoptosis Detection Kit
These assays are for analyzing living cells by facs, fluorescent microscope or with a fluorescent plate reader. For unfixed samples you necrosis can be detected by propidium iodide or Hoechst strain. We use a 488 nm / 530 nm existation emission wavelenght. The samples are cell cultures and tissues. This kits contains stains, wash buffers and dye reagents.
This Annexin V calcium dependent phospholipid binding protein will be downstream of BCL2 pathway and at caspase apoptotic level. Immunochemistry kits has more cell death kits and antibodies.This 1 is suited for programmed cell-death studies.The detections of the targets with this kit is a type of test that can be performed on any target containing biological samples after clean up of interfering agents. The assay must be performed following the protocol.
This Immunochemistry kits Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Immunochemistry kits FITC is highly sensitive to pH extremes.