Human XIAP(Inhibitor Of Apoptosis Protein, X-Linked) ELISA Kit
Size
96TCatalog no#
ELK3429Price
608 EURAssay length
3h
Standard
10ng/mL
Assay Type
Sandwich
Research Area
Apoptosis;
Sensitivity
0.056ng/mL
Detection range
0.156-10ng/mL
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Alternative Names
BIRC4; API3; ILP1; MIHA; XLP2; Baculoviral IAP Repeat-Containing 4; E3 ubiquitin-protein ligase XIAP; IAP-like; Inhibitor of apoptosis protein 3; X-linked inhibitor of apoptosis protein
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Description
This 1 is suited for programmed cell-death studies.Tissue, pathway, proteinase, peptidase, protease ,acrosin, lipoprotein, activator, caspase, trypsin, papain, esterase inhibitors are proteins or receptor ligands or receptor antagonists that bind to an enzyme receptor and decreases its activity. Since blocking an enzyme's activity can kill a pathogen or correct a metabolic imbalance, many drugs are enzyme inhibitors. Not all receptor antagonist that bind to enzymes are inhibitors; enzyme activator ligands or agonists bind to enzymes and increase their enzymatic activity, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme.
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Inhibitor Of Apoptosis Protein, X-Linked (XIAP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Inhibitor Of Apoptosis Protein, X-Linked (XIAP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Inhibitor Of Apoptosis Protein, X-Linked (XIAP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Inhibitor Of Apoptosis Protein, X-Linked (XIAP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.