Rat Factor Related Apoptosis Ligand (FASL) ELISA Kit

Size

96 Tests

Catalog no#

EKU08968

Price

868 EUR

Assay Time

3 hours

Sensitivity

3.4 pg/mL

Shipping Conditions

Ice packs

Detection Method

Colorimetric

Detection Range

7.8-500 pg/mL

Latin name

Rattus norvegicus

Estimated Turnaround Time

6-11 Business days

Species Reactivity

Rattus norvegicus (Rat)

Assay Type

Double-antibody Sandwich

Target Name

Factor Related Apoptosis Ligand

Storage Temperature

Short term: 4°C; Long term: see manual.

Research Area

Apoptosis, Tumor immunity, Infection immunity

Application

For research use only. Not for diagnostic procedures.

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Sample Type

Serum, Plasma, Tissue homogenates and other biological fluids.

Precaution of Use

The Stop Solution is acidic. Do not allow to contact skin or eyes.

Quality Systems

The kit is manufactured at ISO 9001 and ISO 13485 certified facilities.

Shelf life

Use Rat Factor Related Apoptosis Ligand (FASL) ELISA Kit before 8 months

Specificity

This assay has high sensitivity and excellent specificity for detection of Factor Related Apoptosis Ligand

Cross Activity

No significant cross-reactivity or interference between Factor Related Apoptosis Ligand and analogues was observed.

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Test Principle

No significant cross-reactivity or interference between Factor Related Apoptosis Ligand and analogues was observed.

Target's alterntive name

CD178, CD95L, CD95-L, FASLG, FASLG, APT1LG1, APT1-LG1, TNFSF6, Fas Antigen Ligand, TNF Superfamily Member 6, Tumor Necrosis Factor (ligand)Superfamily, Member 6

About

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Factor Related Apoptosis Ligand were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Factor Related Apoptosis Ligand were tested on 3 different plates, 8 replicates in each plate.CV (%) = SD/meanX100;Intra-Assay: CV

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Description

This 1 is suited for programmed cell-death studies.Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.FAS ligand and other ligands are binding to the receptor for signaling pathways for example in apoptosis or JNK signaling. Receptor agonists are often tested for drug development.