Rat Apoptosis Inducing Factor (AIF) ELISA Kit
Size
96 TestsCatalog no#
EKU02533Price
908 EURAssay Time
3 hours
Shipping Conditions
Ice packs
Sensitivity
0.061 ng/mL
Detection Method
Colorimetric
Detection Range
0.156-10 ng/mL
Latin name
Rattus norvegicus
Estimated Turnaround Time
6-11 Business days
Species Reactivity
Rattus norvegicus (Rat)
Assay Type
Double-antibody Sandwich
Target Name
Apoptosis Inducing Factor
Research Area
Signal transduction, Apoptosis
Storage Temperature
Short term: 4°C; Long term: see manual.
Application
For research use only. Not for diagnostic procedures.
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Sample Type
Serum, Plasma, Tissue homogenates and other biological fluids.
Shelf life
Use Rat Apoptosis Inducing Factor (AIF) ELISA Kit before 8 months
Precaution of Use
The Stop Solution is acidic. Do not allow to contact skin or eyes.
Quality Systems
The kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
Target's alterntive name
PDCD8, Programmed Cell Death protein 8, Apoptosis-inducing factor 1, mitochondrial
Specificity
This assay has high sensitivity and excellent specificity for detection of Apoptosis Inducing Factor
Cross Activity
No significant cross-reactivity or interference between Apoptosis Inducing Factor and analogues was observed.
Test Principle
No significant cross-reactivity or interference between Apoptosis Inducing Factor and analogues was observed.
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Apoptosis Inducing Factor were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Apoptosis Inducing Factor were tested on 3 different plates, 8 replicates in each plate.CV (%) = SD/meanX100;Intra-Assay: CV
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Description
This 1 is suited for programmed cell-death studies.Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.