Mouse TRAIL/TNFSF10 (Tumor Necrosis Factor Related Apoptosis Inducing Ligand) CLIA Kit
Size
96TestCatalog no#
E-CL-M0616-96TPrice
541 EURUNIProt ID
NA
Tissue
tumor
Target Species
Mouse
Type
Sandwich
Sensitivity
4.69pg/mL
Detection Type
Colormetric
Latin name
Mus musculus
Target Name
TRAIL/TNFSF10
Detection Range
7.81~500pg/mL
Target Synonym
APO2L, Apo2-L, TL2, CD253
Tested Sample Types
Serum, Plasma, Cell supernatant
Product Name
Mouse TRAIL/TNFSF10 (Tumor Necrosis Factor Related Apoptosis Inducing Ligand) CLIA Kit
Test
MouseĀ orĀ mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Description
This 1 is suited for programmed cell-death studies.Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.FAS ligand and other ligands are binding to the receptor for signaling pathways for example in apoptosis or JNK signaling. Receptor agonists are often tested for drug development.
Test principle
This CLIA kit uses the Sandwich- CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse TRAIL/TNFSF10 . Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TRAIL/TNFSF10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TRAIL/TNFSF10 , biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse TRAIL/TNFSF10 . You can calculate the concentration of Mouse TRAIL/TNFSF10 in the samples by comparing the RLU value of the samples to the standard curve.