NA

Rat

Sandwich

FAS/CD95

7.5pg/mL

Colormetric

12.5~800pg/mL

Rattus norvegicus

Serum, Plasma, Cell supernatant

FASR, TNFRSF6, APO1, APT1, FAS1, FASTM, ALPS1A

Rat FAS/CD95 (Factor Related Apoptosis) CLIA Kit

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

This 1 is suited for programmed cell-death studies.Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.

This CLIA kit uses the Sandwich- CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat FAS/CD95 . Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat FAS/CD95 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat FAS/CD95 , biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat FAS/CD95 . You can calculate the concentration of Rat FAS/CD95 in the samples by comparing the RLU value of the samples to the standard curve.