Recombinant Mouse Apoptosis-mediating Surface Antigen FAS Protein 50µg
Size
50µgCatalog no#
PKSM040905-50µgPrice
376 EURFusion tag
C-6His
Mol Mass
17.4 kDa
AP Mol Mass
25-35 kDa
Expressed Host
Human Cells
Accession
NP_032013.2
Group
recombinants
Latin name
Mus musculus
Sequence
Met 1-Arg 169
Source
Recombinants or rec. proteins
Purity
>95% as determined by reducing SDS-PAGE.
Formulation
Lyophilized from a 0.2 μm filtered solution of PBS,pH7.4.
Description
Antigens are peptides or recombinant or native dependent on the production method.This 1 is suited for programmed cell-death studies.
Synonym
Tumor necrosis factor receptor superfamily member 6; Apo-1 antigen; Apoptosis-mediating surface antigen FAS; FASLG receptor; CD95;Fas;TNFRSF6
Activity
Measured by its ability to inhibit Fas Ligand induced apoptosis of Jurkat human acute T cell leukemia cells.The ED50 for this effect is typically 1-8 μg/mL in the presence of 20 ng/mL recombinant human Fas ligand.
Stability and Storage
Lyophilized protein should be stored at < -20℃, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7℃ for 2-7 days.Aliquots of reconstituted samples are stable at < -20℃ for 3 months
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Background
Mouse Apoptosis-mediating surface antigen FAS (Fas) belongs to the death receptor subfamily of the TNF receptor superfamily and is designated TNFRSF6. Mouse Fas contains 1 death domain and 3 TNFR-Cys repeats. It detected in various tissues including thymus, liver, lung, heart, and adult ovary. As a receptor for TNFSF6/FASLG, The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both.