Mouse Factor Related Apoptosis Ligand (FASL) CLIA Kit, 96 well plate

Size

96T

Catalog no#

EKU08780

Price

1036 EUR

Verified applications

CLIA

Gene number

14103

Protein number

Q544E9

Assay class

Sandwich

Shipping requirements

Blue ice

Use for

8 months

Sensitivity limit

15,5 pg/mL

Latin name

Mus musculus

Assay duration

2 hours, 40min

ELISA detection

Chemiluminescent

Detection limits

41.2-30,000 pg/mL

Estimated production time

7-11 business days

Verified reactivity

Mus musculus (Mouse)

Alternate protein number

Please refer to SwissProt

Gene name

Factor Related Apoptosis Ligand

Research main area

Apoptosis;Tumor immunity;Infection immunity;

Protocol

Please see ELISA's datasheet, otherwise contact us

Notes

For research use only. Not for diagnostic procedures.

QC

The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.

Samples to be analyzed

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

ELISA's cross-reactivity

This assay doesn't seem to cross-react with other species. For more information about cross-reactivity please contact us.

Alternate gene name

CD178; CD95L; CD95-L; FASLG; FASLG; APT1LG1; APT1-LG1; TNFSF6; Fas Antigen Ligand; TNF Superfamily Member 6; Tumor Necrosis Factor(ligand)Superfamily,Member 6

Warnings

Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage recommendation

-20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

ELISA's specificity

This assay has high sensitivity and excellent specificity for detection of Factor Related Apoptosis Ligand (FASL). No significant cross-reactivity or interference between Factor Related Apoptosis Ligand (FASL) and analogues was observed.

Precision of the test

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Factor Related Apoptosis Ligand (FASL) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Factor Related Apoptosis Ligand (FASL) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV

Test

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

ELISA's stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay principle

The microplate provided in this kit has been pre-coated with an antibody specific to Factor Related Apoptosis Ligand (FASL). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Factor Related Apoptosis Ligand (FASL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Factor Related Apoptosis Ligand (FASL) level in the sample or standard.;

Description

This 1 is suited for programmed cell-death studies.Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.FAS ligand and other ligands are binding to the receptor for signaling pathways for example in apoptosis or JNK signaling. Receptor agonists are often tested for drug development.A microtiter plate (spelled Microtiter is a registered trade name in the United States) or microplate or micro well plate or multiwell, is a flat plate with multiple "wells" used as small test tubes. The microplate has become a standard tool in analytical research and clinical diagnostic testing laboratories. A very common usage is in the enzyme-linked immunosorbent assay (ELISA), the basis of most modern medical diagnostic testing in humans and animals. A microplate typically has 6, 24, 96, 384 or 1536 sample wells arranged in a 23 rectangular matrix. Some microplates have even been manufactured with 3456 or 9600 wells, and an "array tape" product has been developed that provides a continuous strip of microplates embossed on a flexible plastic tape.