Apoptosis inhibitor 5 Antibody
WB, IHC-P, IHC-F, ICC
Apoptosis inhibitor 5
Antigen affinity purified
Polyclonal (rabbit origin)
Rabbit (Oryctolagus cuniculus)
0.5mg/ml if reconstituted with 0.2ml sterile DI water
This Apoptosis inhibitor 5 antibodyis to be used only for research purposes and not for diagnostics..
Western blot: 0.5-1ug/ml,IHC (Paraffin): 0.5-1ug/ml,IHC (Frozen): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml
If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
An amino acid sequence from the C-terminus of human Apoptosis inhibitor 5 (amino acids 487-504 of isoform b, KYSSNLGNFNYERSLQGK) was used as the immunogen for this Apoptosis inhibitor 5 antibody.
Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
The stated application concentrations are suggested starting amounts. Titration of the Apoptosis inhibitor 5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
After reconstitution, the Apoptosis inhibitor 5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
Many growth factors and cytokines act as cellular survival factors by preventing programmed cell death(apoptosis). Apoptosis inhibitor 5 (API5) is an antiapoptotic factor which may have a role in protein assembly. The API5 gene to chromosome 11p12 based on an alignment of the API5 sequence with the genomic sequence It is a critical determinant of E2F1-induced apoptosis, acting downstream of E2F to suppress E2F-dependent apoptosis without generally blocking E2F-dependent transcription.
This 1 is suited for programmed cell-death studies.Tissue, pathway, proteinase, peptidase, protease ,acrosin, lipoprotein, activator, caspase, trypsin, papain, esterase inhibitors are proteins or receptor ligands or receptor antagonists that bind to an enzyme receptor and decreases its activity. Since blocking an enzyme's activity can kill a pathogen or correct a metabolic imbalance, many drugs are enzyme inhibitors. Not all receptor antagonist that bind to enzymes are inhibitors; enzyme activator ligands or agonists bind to enzymes and increase their enzymatic activity, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme.