CaspSCREEN Flow Cytometric Apoptosis Detection Kit
Size
25 assaysCatalog no#
K200-25Price
345 EURApplications
NA
Species Reactivity
NA
Storage Conditions
-20°C
Shipping Conditions
gel pack
Sample Type
Live cells
Shelf life
6-12 months
Tag Line
Measures Activated Caspases in intact cells by FACS
Description
This 1 is suited for programmed cell-death studies.The detections of the targets with this kit is a type of test that can be performed on any target containing biological samples after clean up of interfering agents. The assay must be performed following the protocol.
Test
Flow cytometry uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal.