none

+4°C

gel pack

Mammalian

12 months

Biotinylated

Apoptosis Assay Kit

Living cells (suspension and adherent)

• Annexin V-Biotin • 1X Binding Buffer • Propidium Iodide (PI)

Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.

This Annexin V calcium dependent phospholipid binding protein will be downstream of BCL2 pathway and at caspase apoptotic level. Biovision has more cell death kits and antibodies.This 1 is suited for programmed cell-death studies.

• Detection method: Flow cytometry (Ex = 488 nm; Em = 530 nm) using and fluorescence microscopy • Species reactivity: Mammalian • Kit size: Convenient sizes (25, 100, 400 assays) • Applications: Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.

• Simple one staining procedure in 30 minutes • Fast and convenient • Annexin V-Biotin can be detected in conjunction with conventional dye-staining using any streptavidine- or avidin-dye reagents, such as (strept)avidine-fluorescein, -peroxidase, -alkaline phosphatase (AP), and -β-gal, etc.

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.

The Annexin V-Biotin Apoptosis Detection Kit is based on the observation that soon after initiating apoptosis, most cell types translocate the membrane phospholipid phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can easily bind to Biotin-conjugated Annexin V, a protein that has a strong natural affinity for PS. Annexin V-Biotin can be detected in conjunction with conventional dye-staining using any streptavidine- or avidin-dye reagents, such as (strept)avidine-fluorescein, -peroxidase, -alkaline phosphatase (AP), and -β-gal, etc.