Annexin V-Biotin Apoptosis Kit
Size
100 assaysCatalog no#
K109-100Price
480 EURKit's other name
none
Storage condition
+4°C
Shipping condition
gel pack
Species reactivity
Mammalian
Maximum time can be stored
12 months
Conjugation
Biotinylated
Category
Apoptosis Assay Kit
Samples tested
Living cells (suspension and adherent)
Contents
• Annexin V-Biotin • 1X Binding Buffer • Propidium Iodide (PI)
Applications
Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.
Description
This Annexin V calcium dependent phospholipid binding protein will be downstream of BCL2 pathway and at caspase apoptotic level. Biovision has more cell death kits and antibodies.This 1 is suited for programmed cell-death studies.
Highlights
• Detection method: Flow cytometry (Ex = 488 nm; Em = 530 nm) using and fluorescence microscopy • Species reactivity: Mammalian • Kit size: Convenient sizes (25, 100, 400 assays) • Applications: Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.
Kit's benefits
• Simple one step staining procedure in 30 minutes • Fast and convenient • Annexin V-Biotin can be detected in conjunction with conventional dye-staining using any streptavidine- or avidin-dye reagents, such as (strept)avidine-fluorescein, -peroxidase, -alkaline phosphatase (AP), and -β-gal, etc.
Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Kit's description
The Annexin V-Biotin Apoptosis Detection Kit is based on the observation that soon after initiating apoptosis, most cell types translocate the membrane phospholipid phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can easily bind to Biotin-conjugated Annexin V, a protein that has a strong natural affinity for PS. Annexin V-Biotin can be detected in conjunction with conventional dye-staining using any streptavidine- or avidin-dye reagents, such as (strept)avidine-fluorescein, -peroxidase, -alkaline phosphatase (AP), and -β-gal, etc.