Human Apoptosis Inhibitor 5 (API5) ELISA Kit

Size

96 Tests

Catalog no#

EKU08492

Price

885 EUR

Assay Time

3 hours

Shipping Conditions

Ice packs

Research Area

Apoptosis

Sensitivity

0.122 ng/mL

Detection Method

Colorimetric

Detection Range

0.312-20 ng/mL

Estimated Turnaround Time

6-11 Business days

Species Reactivity

Homo sapiens (Human)

Target Name

Apoptosis Inhibitor 5

Assay Type

Double-antibody Sandwich

Storage Temperature

Short term: 4°C; Long term: see manual.

Application

For research use only. Not for diagnostic procedures.

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Sample Type

Tissue homogenates, Cell lysates and other biological fluids.

Shelf life

Use Human Apoptosis Inhibitor 5 (API5) ELISA Kit before 8 months

Precaution of Use

The Stop Solution is acidic. Do not allow to contact skin or eyes.

Quality Systems

The kit is manufactured at ISO 9001 and ISO 13485 certified facilities.

Specificity

This assay has high sensitivity and excellent specificity for detection of Apoptosis Inhibitor 5

Cross Activity

No significant cross-reactivity or interference between Apoptosis Inhibitor 5 and analogues was observed.

Test Principle

No significant cross-reactivity or interference between Apoptosis Inhibitor 5 and analogues was observed.

Target's alterntive name

MIG8, FIF, AAC11, API5L1, XAGL, Cell migration-inducing gene 8, Antiapoptosis clone 11, Fibroblast Growth Factor 2-Interacting Factor 2, Migration-Inducing Protein MIG8

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Apoptosis Inhibitor 5 were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Apoptosis Inhibitor 5 were tested on 3 different plates, 8 replicates in each plate.CV (%) = SD/meanX100;Intra-Assay: CV

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Description

This 1 is suited for programmed cell-death studies.Tissue, pathway, proteinase, peptidase, protease ,acrosin, lipoprotein, activator, caspase, trypsin, papain, esterase inhibitors are proteins or receptor ligands or receptor antagonists that bind to an enzyme receptor and decreases its activity. Since blocking an enzyme's activity can kill a pathogen or correct a metabolic imbalance, many drugs are enzyme inhibitors. Not all receptor antagonist that bind to enzymes are inhibitors; enzyme activator ligands or agonists bind to enzymes and increase their enzymatic activity, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme.