Rabbit polyclonal-Anti-Apoptosis Antagonizing Transcription Factor (AATF)-20ug
Size
20ugCatalog no#
PAA440Mu01-20ugPrice
280 EURImage number
3
Aplication
WB,IHC
Concentration
500ug/ml
Species reactivity
Human,Mouse
Clonality
Rabbit polyclonal
Delivery condition
4℃ with ice bags
Sequence of immunogen
AATF (Ser240~ILe438)
Organism Species
Mus musculus (Mouse)
Latin name
Oryctolagus cuniculus
Group
Polyclonals and antibodies
Source
Polyclonal antibody preparation
Alternative Names
CHE1; DED; Rb-binding protein Che-1
Item Name
Apoptosis Antagonizing Transcription Factor
Applicable Secondary Antibody
SAA544Rb59, SAA544Rb58, SAA544Rb57, SAA544Rb18, SAA544Rb19
Buffer Formulation
PBS, pH7.4,containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Immunogen
RPA440Mu01-Recombinant Apoptosis Antagonizing Transcription Factor (AATF)
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Storage instructions
Avoid repeated freeze/thaw cycles. Store at 4 ℃ for frequent use. Aliquot and store at -20℃ for 12 months.
About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by Cloud Clone Corp. Rabbit antibodies are very stable and can be stored for several days at room temperature. Cloud Clone Corp adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.This 1 is suited for programmed cell-death studies.Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.The activation of transcription factor subunits is the first step of gene expression, in which a particular segment of DNA is copied into RNA (mRNA) by the enzyme RNA polymerases. Transcription factors, unites and elongations can be RNA and DNA nucleic acids, base pairs of nucleotides . Converting from DNA to RNA is made by enzymatic reactions. During transcription, a DNA sequence is read by an RNA polymerase, which produces a complementary, anti-parallel RNA strand called a primary transcript. Transcriptions are key functions in signal transduction pathways. Signaling ligand binding transcription factors play an important role in transduction cascades.