UNIProt ID

NA

Target Species

Human

Type

Sandwich

Target Name

FAS/CD95

Sensitivity

9.38pg/mL

Detection Type

Colormetric

Detection Range

15.63~1000pg/mL

Tested Sample Types

Serum, Plasma, Cell supernatant

Target Synonym

FASR, TNFRSF6, APO1, APT1, FAS1, FASTM, ALPS1A

Product Name

Human FAS/CD95 (Factor Related Apoptosis) CLIA Kit

Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Description

This 1 is suited for programmed cell-death studies.Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.

Test principle

This CLIA kit uses the Sandwich- CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human FAS/CD95 . Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FAS/CD95 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FAS/CD95 , biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human FAS/CD95 . You can calculate the concentration of Human FAS/CD95 in the samples by comparing the RLU value of the samples to the standard curve.