NA

NA

Porcine

Sandwich

14.06pg/mL

FASL/TNFSF6

Colormetric

23.44~1500pg/mL

Serum, Plasma, Cell supernatant

MeSH E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay

Porcine FASL/TNFSF6(Factor Related Apoptosis Ligand) EIA Kit

This 1 is suited for programmed cell-death studies.This ELISA test kit or EIA Enzyme immuno assay is an enzyme linked immuno assay supplied as coated 96 well plates with antigen or antibody and needs to be stored at +4°C.Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.FAS ligand and other ligands are binding to the receptor for signaling pathways for example in apoptosis or JNK signaling. Receptor agonists are often tested for drug development.

This EIA kit uses the Sandwich-EIA principle. The micro EIA plate provided in this kit has been pre-coated with an antibody specific to Porcine FASL/TNFSF6. Standards or samples are added to the micro EIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Porcine FASL/TNFSF6 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Porcine FASL/TNFSF6, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Porcine FASL/TNFSF6. You can calculate the concentration of Porcine FASL/TNFSF6 in the samples by comparing the OD of the samples to the standard curve.